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Evaluation of aortic changes in elderly people autopsied with acquired immunodeficiency syndrome
Aortic changes in elderly people with AIDS
Surgical and Experimental Pathology volume 1, Article number: 7 (2018)
To verify the distribution of the degree of fat deposits and percentage of collagen and elastic fibers in the intimal and medial layers of the aorta of elderly patients autopsied, with and without Acquired Immunodeficiency Syndrome (AIDS).
Twenty-six fragments of the aorta from elderly patients autopsied in the period from 1982 to 2014, were collected and, divided into two groups, being 13 with AIDS and 13 without AIDS. The intensity of the atherosclerosis was macroscopically evaluated in a semi-quantitative way. To quantify the elastic and collagen fibers, it was used the Leica Qwin Plus® software.
In the comparison of the macroscopic intensity of atherosclerosis, the AIDS group (t = 0.6969, p = 0.4992). Regarding elastic fibers, there was a significant increase in the AIDS group (U = 115,800; p < 0.0001). Comparing the groups, there was a significant increase of the collagen fibers in the aortic layers in the AIDS group (U = 114,100; p < 0.0001).
The infection by the HIV influence in the amount of fibers in the extracellular matrix and the intensity of lipid deposit, demonstrating that it may be a risk factor to be considered for the development of atherosclerosis.
According to the Ministry of Health, in Brazil there is more than 39 million people aged 50 years or more (Ministério da Saúde [homepage on the Internet] 2012). Considering the confirmation of the Human Immunodeficiency Virus (HIV), individuals in this age range are regarded elderly people (Center For Disease Control (CDC) [homepage on the Internet] 2002).
Described for the first time in 1981, Acquired Immunodeficiency Syndrome (AIDS), disease resulting from HIV infection, is a great problem of public health around the world, causing immunosuppression and consequently opportunistic infections and neoplasia (Center For Disease Control (CDC) [homepage on the Internet] 2002).
The number of elderly people coexisting with HIV has increased and some of the reasons related to such increase are the progressive cultural changes in society, with regard to sexuality, low adhesion to the use of condom, dissemination of drug therapy with antiretroviral drugs for AIDS and the progressive studies in the area that contributed for the reduction in the mortality rates of the contaminated individuals (Verolet et al. 2015; Serra et al. 2013; Frugoli and Magalhães Júnior 2011; Ray et al. 2010). Associated to this, in elderly people, the atherosclerosis presents higher indices of occurrence, due to the characteristics resulting from ageing and the exposure time to risk factors (Pires et al. 2004).
Atherosclerosis is a chronic cardiovascular disease (Ketelhuth and Hansson 2015) which affects blood vessels, mainly the medium and large caliber elastic arteries (Krause et al. 2007), such as the aorta. Its etiology is multifactorial with genetic and environmental influences (Perk et al. 2012). The injuries, resulting from this process, are related to an excessive fibrous and proliferative inflammatory response to different forms of aggression against the endothelium and the smooth muscle in the artery walls. A great number of growth factors, cytokines and vessel-regulator molecules participate in this process (Ross 1993).
In this context, the goal of this study was to analyze the intensity of atherosclerosis and the percentage of elastic and collagen fibers in the intimal and medial layers of the aorta artery of elderly patients autopsied, with and without AIDS. With this, the study aims to contribute for a better understanding of the association between the retrovirus and the arterial remodeling and the development of atherosclerosis, in elderly patients.
This is a retrospective study approved by the Research Ethics Committee of the Federal University of Minas Gerais Triangle (CEP-UFTM) under the protocol number 1376. We used twenty-six cases, with more than 50 years old, autopsied at a hospital of Uberaba, Minas Gerais, Brazil, from 1982 to 2014. Two pathologists carried out the autopsy and the diagnosis of AIDS was made through the evidence of at least one of the defining diseases of the syndrome and by the cluster of differentiation 4 (CD4) count below 200 cells/mm32. Data relating to age, sex, cause of death and color were collected from the reports of the autopsies. Regarding the use of antiretroviral therapy, data were available from the pharmacy department. The fragments were retrieved from the archive of anatomical specimens of the Discipline of General Pathology.
Of the 141 segments analyzed, 13 were selected from patients who were 50 years of age or older who were diagnosed with AIDS and were viable for the analysis, independent of gender, color, cause of death or base disease. With the objective of pairing with the study group, 13 segments were collected and constituted the group without AIDS. Patients aged 50 years or older were considered elderly (Center For Disease Control (CDC) [homepage on the Internet] 2002; Cardoso et al. 2013; Favarato et al. 2016).
The aorta fragments were evaluated with the help of a standardized scale of 12.0 cm. Each examiner measured the degree of fatty deposit on the non-millimetric scale, subjectively measuring one point on the scale. Next, with the help of a ruler, it was measured the distance between 0.0 cm and the point marked on the scale.
Consensually, the observers classified the atherosclerosis according to the degree of fatty deposit into mild, from 0.1 cm to 4.0 cm, moderate, from 4.1 cm to 7.0 cm, and severe from 7.1 cm to 12.0 cm. The degree of fatty deposit in the macroscopy was evaluated according to the classification into mild, moderate or severe (Fig. 1). From this criterion, the observers of this study described the atherosclerosis quantitatively. For this evaluation, the extension of the atheromatous plaques, presence of fibrosis and calcification, were considered (Ferraz et al. 2012).
All fragments were subjected to histological processing, and then serial cuts of 4 μm thick were made for histochemical procedures.
In the verhoeff stain, it was observed the elastic fibers in the intimal and medial layers of the abdominal portion of the aorta artery, viewed under common light and 40x objective with total magnification of 1250x. The picrosirius staining was used to quantify the collagen fibers in the intimal and medial layers of the abdominal portion of the aorta artery, examined under polarized light, with 40x objective and total magnification of 1250x, through four quadrants and subsequent division of 10 fields in both staining. The Leica Qwin Plus® software for morphometry made the analyses of the collagen and elastic fibers (Fig. 2).
For the statistical analysis, it was elaborated a Microsoft Excel® spreadsheet. The information was analyzed using the software GraphPad Prism® 5. To verify the distribution of the variables, it was applied a Shapiro-Wilk and Kolmogorov-Smirnov statistical test. The t student test was used for normal distribution for the comparison of two groups and the Mann-Whitney test (U) was used for non-normal distribution for the comparison of two groups. As for the correlation, it was applied the Spearman (rS) for non-normal distribution. The differences were considered significant when significance level (p) was lower than 5% (p < 0.05).
In the present study, 26 fragments of the abdominal aorta from elderly patients autopsied, with and without AIDS, were obtained and analyzed.
Data relating to age, sex and color, were described in Table 1.
With regard to the classifications of cause of death, data were presented in Table 2.
According to the use of antiretroviral therapy, the results are described in Table 3.
In the comparison of the macroscopic intensity of atherosclerosis, the AIDS group presented higher intensity when compared to the non-AIDS group (t = 0.6969, p = 0.4992) (Table 4).
In relation to elastic fibers, there was a significant increase in the group with AIDS compared to that without AIDS (U = 115,800; p < 0.0001) (Fig. 3).
Comparing the groups, there was a significant increase of the collagen fibers in the intimal and medial layers of the aorta in the AIDS group compared to that without AIDS (U = 114,100; p < 0.0001) (Fig. 4).
Evaluating the percentage of elastic fibers in the intima and medial layers of the aorta artery with age, there was a negative correlation (rS = − 0.0438; p = 0.3182) in the AIDS group.
Analyzing the group with AIDS, there was a positive correlation between the advancing age and the increase of collagen fibers in the intima and medial aortic artery layers (rS = 0.0387; p = 0.3776).
In the evaluation of the percentage of elastic fibers with age, there was a positive and significant correlation (rS = 0.1507; p = 0.0006) in the group without AIDS.
In the non- AIDS group, the correlation between collagen fiber percentage and age was negative and significant (rS = − 0.1725; p < 0.0001).
All the graphs of correlation analysis are showed on Fig. 5.
The present study compared through the semi-quantitative and morphometric methods, the intensity of atherosclerosis and the percentage of elastic and collagen fibers in the intimal and medial layers of the aorta artery of autopsied elderly people with and without AIDS.
The life expectancy for the patients with HIV, after starting the use of anti-retroviral therapy increased considerably (Kramer et al. 2009). Despite the increase in the life expectancy and greater exposure of elderly people contaminated to risk factors for cardiovascular diseases, we verified that in the group with AIDS, the cause of death was predominantly infectious (76.92%) and in the group without AIDS, the majority was related to cardiovascular events (53.85%).
In the macroscopic evaluation of the aorta, we can observe that the AIDS group had higher atherosclerosis intensity than the non-AIDS group. In the literature, a study performed through autopsy analysis reports on endothelial dysfunction in HIV-positive individuals, such as multiple vessel lesions and muscle cell hyperplasia (Chan et al. 2009), with endothelial dysfunction due to increased lipids in the vessel leads to the entry of lipoproteins in the intima layer forming lipidic streaks, an initial manifestation of atherosclerosis (Xavier 2013; Hansson 2009).
In the group with AIDS, elastic fibers and collagen fibers showed a significant increase when compared to the group without AIDS. With the progression of AIDS, the components of the extracellular matrix are altered, which causes an increase in collagen fibers, elastic fibers, laminin, fibronectin and proteoglycans (Paiva et al. 1996; Leng SX Yang and Walston 2004; De Lima Pereira et al. 2007). Moreover, it is demonstrated that the disease is associated with a higher percentage of collagen fibers, related to the greater activation of the immune system in a chronic way and with local inflammation (Shacker et al. 2005; Estes et al. 2007).
In the evaluation of the percentage of elastic fibers in the intima and medial layers of the aorta with age, the AIDS group showed a negative correlation and the group without AIDS, a positive and significant correlation. Due to the AIDS patients, were more marked with the effects of atherosclerosis, changes in the amount of elastic fibers occur due to the damages suffered in the extracellular matrix of the disease (Kurtz and Oh 2012), in the atherogenesis the action of enzymes promote the degradation of elastic fibers and, with ageing, decreased elastin synthesis (Wachi 2011). Other aggravating factors are calcifications that occur in atherosclerotic plaques, that may affect from fibers, due to calcification of elastin, which results in complete degradation (Bobryshev 2005).
There was a positive and significant correlation between the increase in the percentage of collagen fibers and the advancing age of patients with AIDS. The vascular remodeling initiated by the inflammation, when in the presence of HIV, can be linked to the infection itself or metabolic factors associated (Ho and Hsue 2009). The event is triggered by activation of the endothelial cells and increased expression of collagen (Crowe et al. 2010). This group had a higher intensity of atherosclerosis, with the formation of atherosclerotic plaques triggers an inflammatory process that involves the smooth muscle cells of the intimal layer secreting collagen, elastic fibers and proteoglycans for the extracellular matrix (Insull 2009).
The non-AIDS group demonstrated a negative and significant correlation between aging and collagen fibers. The results corroborate with a literature that shows that with aging there is loss of collagen fibers as a consequence of the synthesis reduction and increase of its degradation (Chung et al. 2001).
As the age increases, there is a greater exposure to risk factors related to the development of atherosclerosis, which may lead to arterial remodeling, one of which is the development of AIDS, as demonstrated in the literature and in the present study.
Advances in the treatment of AIDS are progressively increasing the life expectancy among those infected by the virus, being a positive factor for public health. However, the findings in this study suggest that HIV infection may influence the greater development of arterial remodeling and consequently atherosclerosis, especially in the elderly population due to age being associated with atherogenesis due to the longer time of exposure to aggressors and aging processes.
Acquired Immunodeficiency Syndrome
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- CD4 :
Cluster of differentiation 4
Committee of the Federal University of Minas Gerais Triangle
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Human Immunodeficiency Virus
Triângulo Mineiro Federal University
Bobryshev YV (2005) Calcification of elastic fibers in human atherosclerotic plaque. Atherosclerosis 180(2):293–303
Cardoso SW, Torres TS, Santini-Oliveira M, Marins LMS, Veloso VG, Grinsztejn B (2013) Aging with HIV: a practical review. Braz J Infect Dis
Center For Disease Control (CDC) [homepage on the Internet] (2002) Deaths among persons with AIDS through. HIV Surveillance Rep 8:1
Chan W, Sviridov D, Dart AM (2009) HIV, atherosclerosis and inflammation: implications for treatment. J HIV Ther 14:61–68
Chung JH, Seo JY, Choi HR, Lee MK, Choon SY, Rhie G et al (2001) Modulation of skin collagen metabolism in aged and Photoaged human skin in vivo. J Invest Dermatol 117(5):1218–1224
Crowe SM, Westhorpe CL, Mukhamedova N, Jaworowski A, Sviridov D, Burkinsky M (2010) The macrophage: the intersection between HIV infection and atherosclerosis. J Leukoc Biol 87:589–598
De Lima Pereira SA, Rodrigues DBR, Castro ECC, Reis MA, Teixeira VPA (2007) Quantitative analysis of fibrosis and mast cells in the tongue of chronic chagasic patients: autopsy study. Med Mal Infect 27(4):229–233
Estes JD, Wietgrefe S, Schacker T, Southern P, Beilman G, Reilly C et al (2007) Simian immunodeficiency virus-induced lymphatic tissue fibrosis is mediated by transforming growth factor beta 1-positive regulatory T cells and begins in early infection. J Infect Dis 195(4):551–561
Favarato GKNA, Souza ACS, Oliveira LF, Ferraz MLF, Teixeira VPA, Cavellani CLC (2016) Skin aging in patients with acquired immunodeficiency syndrome. Ann Diagn Pathol 24:35–39
Ferraz MLF, Nascimento DMS, Rorato JPH, Espindula AP, Oliveira LF, Ramalho LS et al (2012) Correlation of lifetime progress of atherosclerosis and morphologic markers of severity in humans: new tools for a more sensitive evaluation. Clinics 67:1071–1075
Frugoli A, Magalhães Júnior CAO (2011) A sexualidade na terceira idade na percepção de idosas e indicações para a educação sexual. Arq Cienc Saúde 15:83–95
Hansson GK (2009) Atherosclerosis – an immune disease – the Anitschkov lecture 2007. Atherosclerosis 202:2–10
Ho JE, Hsue PY (2009) Cardiovascular manifestations of HIV infection. Heart 95:1193–1202
Insull JR (2009) The pathology of atherosclerosis: plaque development and plaque responses to medical treatment. Am J Med 122:s3–s14
Ketelhuth DF, Hansson GK (2015) Modulation of autoimmunity and atherosclerosis - common targets and promising translational approaches against disease. Circ J 79:924–933
Kramer AS, Lazzarotto AR, Sprinz E, Manfroi WC (2009) Alterações metabólicas, terapia antirretroviral e doença cardiovascular em idosos portadores de HIV. Arq Bras Cardiol 93:561–568
Krause MP, Hallage T, Gama MPR, Sasaki JE, Miculis CP, Buzzachera CF, Silvas SG (2007) Association between lipid profile and adiposity in women over age 60. Arq Bras Cardiol 89:147–153
Kurtz A, Oh S (2012) Age related changes of the extracellular matrix and stem cell maintenance. Prev Med 54:s50–s56
Leng SX Yang H, Walston JD (2004) Decreased cell proliferation and altered cytokine production in frail older adults. Exp Gerontol 16(3):249–252
Ministério da Saúde [homepage on the Internet] (2012) Secretaria Executiva. Datasus: Informações de Saúde. Demográficas e socioeconômicas Avaiable from: http://www.datasus.gov.br
Paiva DD, Morais JC, Pilotto J, Veloso V, Duarte F, Lenzi HL (1996) Spectrum of morphologic changes of 16 lymph nodes in HIV infection. Mem Inst Oswaldo Cruz 91(3):371–379
Perk J, De Backer G, Gohlke H, Graham I, Reiner Z, Verschuren M et al (2012) European guidelines on cardiovascular disease prevention in clinical practice. The fifth joint task force of the European Society of Cardiology and Other Societies on cardiovascular disease prevention in clinical practice. Eur Heart J 33:1635–1701
Pires SL, Gagliardi RJ, Gorzoni ML (2004) Estudo das frequências dos principais fatores de risco para acidente vascular cerebral isquêmico em idosos. Arq Neuropsiquiatr 62:844–851
Ray M, Logan R, Sterne JA, Hernandez-Diaz S, Robins JM, Sabin C et al (2010) The effect of combined antiretroviral therapy on the overall mortality of HIV-infected individuals. AIDS 24:123–137
Ross R (1993) The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362:801–809
Serra A, Sardinha AHL, Pereira ANS, Lima SCVS (2013) Percepção de vida dos idosos portadores do HIV/AIDS atendidos em centro de referência estadual. Saúde em debate 97:294–304
Shacker TW, Reilly C, Beilman GJ, Taylor J, Skarda D, Krason D et al (2005) Amount of lymphatic tissue fibrosis in HIV infection predicts magnitude of HAART-associated change in peripheral CD4 cell count. AIDS 19(18):2169–2171
Verolet CM, Delhumeau-Cartier C, Sartori M, Toma S, Zawadynski S, Becker M et al (2015) Lipodystrophy among HIV-infected patients: a cross-sectional study on impact on quality of life and mental health disorders. AIDS Res Ther 12:21
Wachi H (2011) Role of elastic fiebers on cardiovascular disease. J Health Sci 57:449–457
Xavier HT (2013) V Diretriz Brasileira de Dislipidemias e Prevenção da Aterosclerose. Arq Bras Cardiol 101:1–22
The present study was supported by National Council for Scientific and Technological Development (CNPq), Coordination of Improvement of Higher Education Personnel (CAPES), Foundation for Research Support of the State of Minas Gerais (FAPEMIG) and Fundação Of Teaching and Research of Uberaba (FUNEPU).
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The datasets used and analyzed during this study are available from the corresponding author on reasonable request.
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This study was approved by the Research Ethics Committee of the Federal University of Minas Gerais Triangle (CEP-UFTM) under the protocol number 1376.
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Oliveira, M.S., Torquato, B.G.S., da Silveira, L.A.M. et al. Evaluation of aortic changes in elderly people autopsied with acquired immunodeficiency syndrome. Surg Exp Pathol 1, 7 (2018). https://doi.org/10.1186/s42047-018-0025-4
- Acquired immunodeficiency syndrome
- Elderly people