Fig. 3

Balloon cells, dysmorphic and hypertrophic neurons presented characteristics of undifferentiated and mature cells. BCs were decorated by antibodies against nestin (a), vimentin (b; arrow in n) and TUJ-1 (c), which are usually expressed by undifferentiated neural cells. BCs expressed GFAP at a variable intensity or not at all (d). BCs were also marked by antibodies against neurofilament proteins (arrow in e), MAP2 (f), β-tubulin (g), pan-neuronal filaments (h), and NeuN (arrow, i). DNs were stained by anti- β-tubulin (j), SMI 311 (k), and anti-NeuN (arrowheads in l). DNs were also marked by anti-nestin (m), anti-vimentin (n), and anti- neuronal class III β-tubulin (o). HyNs were stained with the anti-NeuN antibody (arrowhead in i). Cases: 10, a, c, d, e, g, h, i, j, k, l, o; 9, b, m, n; 5, f. Bars, 30 μm for all panels, except for panel (d), 50 μm