Fig. 5

Immunohistochemical evaluation. a Invasion (arrow) of non-necrotic muscle fiber by cytotoxic/ supressor CD8 positive T lymphocytes in a patient with sporadic inclusion body myositis. b Abnormal sarcolemmal positivity for major histocompatibility complex class I (MHC-I) in patient with dermatomyositis (inset shows normal control with blood vessel wall reaction). c Intrasarcoplasmic desmin reactive (arrow) material in patient with myofibrillar myopathy. d Ectopic intrasarcoplasmic dystrophin expression in patient with myofibrillar myopathy. e Complete immunohistochemical dystrophin deficiency in patient with dystrophinopathy type Duchenne muscular dystrophy (inset shows normal control). f Decreased dystrophin reaction in patient with dystrophinopathy type Becker muscular dystrophy. g. and h Serial sections showing abnormal utrophin reaction (g) in sarcoplasmic membranes of some fibers (asterisks) without signs of regeneration, negative for neonatal myosin (h) in patient with dystrophinopathy type Becker (insets are normal controls). i and j. serial sections of negative fibers in mosaic pattern in fibers without necrosis (with sarcolemma positive for spectrin in j) in a female symptomatic carrier of dystrophinopathy. k. normal emerin immunophenotypic expression in nuclear membrane (arrow). l. normal sarcomeric (intrasarcoplasmic) telethonin expression (arrow). a. CD8 400x, b. MHC-I 200x (inset normal control original magnification MHC-I 200x), c. desmin 200x, d. dystrophin carboxy-terminal DYS2 200x, e. dystrophin carboxy-terminal DYS2 200x, f. dystrophin amino-terminal DYS3 200x, g. utrophin 100x, h. neonatal myosin 100x, i. dystrophin amino-terminal DYS3 100x, j. spectrin 100x, k. emerin 200x, l. telethonin 200x