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Table 1 Microsatellite STR results of the eight samples examined. Samples without contamination present only alleles for each marker, for example, sample 1, presents only two alleles for the marker D3S1358, with each allele having 16 and 18 replicates. Samples considered contaminated have more than two alleles per marker, for an instance, sample 3 has 3 alleles for marker D3S1358 (15, 16 and 17), sample 5 has 3 alleles (5, 7, 9.3) for marker TH01. L: DNA extracted from the slides; B: DNA extracted from the block; (n) Allele number of each marker; “-”, amplification not available

From: STR technique for the detection of contamination by exogenous DNA in paraffin blocks and histological slides

Markers

Sex

Autossomic

amel

D3S1358

TH01

D21S11

D18S51

Penta E

D5S818

D13S327

D7S820

D16S539

CSF1PO

Penta D

vWA

D8S1179

TPOX

FGA

Size

100-200pb

100-200pb

100-200pb

201-300pb

301-400pb

301-400pb

100-200pb

100-200pb

201-300pb

301-400pb

301-400pb

301-400pb

100-200pb

201-300pb

201-300pb

301-400pb

Samples

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

L

B

1

XY

X

(16) (18)

(16) (18)

(7)

(7)

(29) (33)

(29) (33)

(13) (16)

(13) (16)

(10)

(10)

(10)

(14)

(14)

(9) (11)

(9) (11)

(10)

(10)

(11) (12)

(11) (12)

(13)

(11)

(14) (16)

(14) (16)

(13)

(13)

(6) (8)

(6) (8)

(23)

(23) (25)

2

XY

XY

(14) (16)

(14) (15) (17)

(6) (8)

(6) (7) (8) (9)

(28)

(13) (15)

(12)

(12)

(12) (13)

(9) (11)

(10)

(12) (13)

(11)

(6) (11)

(12) (14) (16) (17) (18)

(16) (18)

(13) (14)

(8) (11)

(20)

(23) (24)

3

XY

XY

(15)

(15) (16) (17)

(9.3)

(6) (8)

(29)

(14)

(13)

(9) (16)

(13)

(12)

(11)

(10) (13)

(13) (14) (15) (18) (19)

(16) (17)

12

(8)

(19) (20) (25)

4

XY

X

(16)

(14) (15)

(7) (9.3)

(29)

(14)

(14) (18)

(9) (16)

(11)

(7) (11)

(13)

(9) (11)

(11)

(10) (11)

(9)

(9) (10)

(11) (12)

(10)

(9) (10)

(14) (18)

(13)

(11) (13)

(9)

(8)

(23)

(20) (23)

5

XY

X

(16)

(16)

(5) (7) (9.3)

(9.3)

(29)

(31.2)

(13)

(9)

(10) (11)

(10) (11)

(9) (10) (11)

(11) (12)

(10)

(8)

(9) (11)

(10)

(12)

(2.2)

(15) (16) (18) (19)

(16) (19)

(8)

(9)

(20) (23)

6

XY

XY

(14) (15) (16) (17) (18)

(15) (18)

(7) (9.3)

(6)

(21)

(14) (15) (18) (19)

(10) (11)

(12)

(9) (12)

(15) (10) (17) (18)

(11)

7

XY

XY

(16) (17)

(15)

(6) (9.3)

(6)

(29) (31.2)

(17)

(13)

(11) (13)

(7) (8) (10) (11)

(11)

(9) (14)

(11)

(11)

(14)

(14) (15) (16)

(11) (15)

(8)

8

XY

X

(17)

(17)

(9.3)

(8)

(16)

(11) (12)

(12)

(11) (13)

(16)

(16) (20)

(14)