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Table 1 Antibodies used in the present immunohistochemistry assays

From: Identification and immunophenotype of abnormal cells present in focal cortical dysplasia type IIb

Antibody

Host

aDilution

Source/catalog

Immunogen

Description

Primary antibodies

 Anti-glial fibrillary acidic protein

Rabbit

1:500

Dako/Z0334

Bovine glial fibrillary acidic protein (GFAP)

Astrocyte marker

 Anti-vimentin, clone V9

Mouse

1:500

Dako/M0725

Pig vimentin

Mesenchymal cell marker

 Anti-nestin, clone rat-401

Mouse

1:100

Chemicon/MAB353

Rat nestin

Marker of intermediate filament protein (class type VI) expressed during development

 Anti-β-tubulin, clone N357

Mouse

1:200

Amersham/N357

β-tubulin

Microtubules marker

 Anti-tubulin, neuronal class III β-tubulin, clone TU-20

Mouse

1:50

Millipore/MAB1637

Synthetic peptide corresponding to amino acids 443–450 of human class III beta-tubulin conjugated to KLH

Reacts with the C-terminus of the neuron specific β-III isoform of tubulin

 Anti-NeuN, clone A60

Mouse

1:200

Chemicon/MAB377

Purified cell nuclei from mouse brain

Marker of neuron-specific nuclear protein

 Anti-neurofilament protein, clone 2F11

Mouse

1:100

Dako/M0762

Neurofilament M isolated from normal adult human brain

Neuron marker

 Antibody against pan-neuronal neurofilament

Mouse

1:800

Covance/SMI-311

Neuron intermediate filaments

Neuron marker

 Anti-microtubule associated protein-2, clone AP20

Mouse

1:200

Millipore/MAB3418

Bovine brain microtubule protein-2

Marker of neuronal cell bodies and dendrites

Secondary antibodies

 Anti-mouse IgG

Rabbit

1:100

Dako/E0354

Rabbit IgG conjugate biotin

 Anti-rabbit IgG

Pig

1:100

Dako/E0353

Pig IgG conjugate biotin

  1. aAll antibodies were diluted in 50 mM sodium phosphate buffer, pH 7.5, containing 0.09% (weight/volume) NaCl, 0.03% (volume / volume) Triton X-100, 3% (weight/volume) bovine serum albumin and 15% (volume / volume) goat serum