From: Identification and immunophenotype of abnormal cells present in focal cortical dysplasia type IIb
Antibody | Host | aDilution | Source/catalog | Immunogen | Description |
---|---|---|---|---|---|
Primary antibodies | |||||
Anti-glial fibrillary acidic protein | Rabbit | 1:500 | Dako/Z0334 | Bovine glial fibrillary acidic protein (GFAP) | Astrocyte marker |
Anti-vimentin, clone V9 | Mouse | 1:500 | Dako/M0725 | Pig vimentin | Mesenchymal cell marker |
Anti-nestin, clone rat-401 | Mouse | 1:100 | Chemicon/MAB353 | Rat nestin | Marker of intermediate filament protein (class type VI) expressed during development |
Anti-β-tubulin, clone N357 | Mouse | 1:200 | Amersham/N357 | β-tubulin | Microtubules marker |
Anti-tubulin, neuronal class III β-tubulin, clone TU-20 | Mouse | 1:50 | Millipore/MAB1637 | Synthetic peptide corresponding to amino acids 443–450 of human class III beta-tubulin conjugated to KLH | Reacts with the C-terminus of the neuron specific β-III isoform of tubulin |
Anti-NeuN, clone A60 | Mouse | 1:200 | Chemicon/MAB377 | Purified cell nuclei from mouse brain | Marker of neuron-specific nuclear protein |
Anti-neurofilament protein, clone 2F11 | Mouse | 1:100 | Dako/M0762 | Neurofilament M isolated from normal adult human brain | Neuron marker |
Antibody against pan-neuronal neurofilament | Mouse | 1:800 | Covance/SMI-311 | Neuron intermediate filaments | Neuron marker |
Anti-microtubule associated protein-2, clone AP20 | Mouse | 1:200 | Millipore/MAB3418 | Bovine brain microtubule protein-2 | Marker of neuronal cell bodies and dendrites |
Secondary antibodies | |||||
Anti-mouse IgG | Rabbit | 1:100 | Dako/E0354 | Rabbit IgG conjugate biotin | – |
Anti-rabbit IgG | Pig | 1:100 | Dako/E0353 | Pig IgG conjugate biotin | – |