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Table 1 Antibodies used in the present immunohistochemistry assays

From: Identification and immunophenotype of abnormal cells present in focal cortical dysplasia type IIb

Antibody Host aDilution Source/catalog Immunogen Description
Primary antibodies
 Anti-glial fibrillary acidic protein Rabbit 1:500 Dako/Z0334 Bovine glial fibrillary acidic protein (GFAP) Astrocyte marker
 Anti-vimentin, clone V9 Mouse 1:500 Dako/M0725 Pig vimentin Mesenchymal cell marker
 Anti-nestin, clone rat-401 Mouse 1:100 Chemicon/MAB353 Rat nestin Marker of intermediate filament protein (class type VI) expressed during development
 Anti-β-tubulin, clone N357 Mouse 1:200 Amersham/N357 β-tubulin Microtubules marker
 Anti-tubulin, neuronal class III β-tubulin, clone TU-20 Mouse 1:50 Millipore/MAB1637 Synthetic peptide corresponding to amino acids 443–450 of human class III beta-tubulin conjugated to KLH Reacts with the C-terminus of the neuron specific β-III isoform of tubulin
 Anti-NeuN, clone A60 Mouse 1:200 Chemicon/MAB377 Purified cell nuclei from mouse brain Marker of neuron-specific nuclear protein
 Anti-neurofilament protein, clone 2F11 Mouse 1:100 Dako/M0762 Neurofilament M isolated from normal adult human brain Neuron marker
 Antibody against pan-neuronal neurofilament Mouse 1:800 Covance/SMI-311 Neuron intermediate filaments Neuron marker
 Anti-microtubule associated protein-2, clone AP20 Mouse 1:200 Millipore/MAB3418 Bovine brain microtubule protein-2 Marker of neuronal cell bodies and dendrites
Secondary antibodies
 Anti-mouse IgG Rabbit 1:100 Dako/E0354 Rabbit IgG conjugate biotin
 Anti-rabbit IgG Pig 1:100 Dako/E0353 Pig IgG conjugate biotin
  1. aAll antibodies were diluted in 50 mM sodium phosphate buffer, pH 7.5, containing 0.09% (weight/volume) NaCl, 0.03% (volume / volume) Triton X-100, 3% (weight/volume) bovine serum albumin and 15% (volume / volume) goat serum