Title | Age | Authors | Argumentation |
---|---|---|---|
Comparison of an in vitro Diagnostic Next-Generation Sequencing Assay with Sanger Sequencing for HIV-1 Genotypic Resistance Testing. | 2018 | Tzou PL, et al. (2018) | Sensitivity of this method is insufficient for identifying low frequency mutations. |
A simple and robust real-time qPCR method for the detection of PIK3CA mutations. | 2018 | Alvarez-Garcia V; et al... (2018) | Low sensitivity and the high cost. |
Droplet Digital PCR for Mutation Detection in Formalin-Fixed, Paraffin-Embedded Melanoma Tissues: A Comparison with Sanger Sequencing and Pyrosequencing. | 2018 | McEvoy AC; et al. (2018) | Failure to detect mutations in genes and specific samples. |
Implementation of next generation sequencing technology for somatic mutation detection in routine laboratory practice. | 2018 | Giardina T; et al. (2018) | It presents greater difficulties in detecting materials that were not microdissected. |
Non-reproducible sequence artifacts in FFPE tissue: an experience report. | 2017 | Ofner R, et al. (2017) | Low reproducibility in FFPE. |
Clinical validation of the 50 gene AmpliSeq Cancer Panel V2 for use on a next generation sequencing platform using formalin fixed, paraffin embedded and fine needle aspiration tumour specimens. | 2017 | Rathi V; et al. (2017) | Disadvantages in relation to new technologies such as NGS |
Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis. | 2016 | Ishige T; et al (2016) | Sensitivity of this method is insufficient for identifying low frequency mutations. |
Clinical Applications of Next-Generation Sequencing in Cancer Diagnosis | 2016 | Sabour L; et al. (2017) | Laborious, time consuming and difficulty in distinguishing between normal and altered genotypes. |
A combination of immunohistochemistry and molecular approaches improves highly sensitive detection of BRAF mutations in papillary thyroid cancer. | 2016 | Martinuzzi C; et al. (2016) | Low sensitivity |