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Table 3 Shows some recent articles arguing about advantages and disadvantages of Sanger sequencing in relation to other methods

From: KIT exon 11 and PDGFRA exon 18 gene mutations in gastric GIST: proposal of a short panel for predicting therapeutic response

Title

Age

Authors

Argumentation

Comparison of an in vitro Diagnostic Next-Generation Sequencing Assay with Sanger Sequencing for HIV-1 Genotypic Resistance Testing.

2018

Tzou PL, et al. (2018)

Sensitivity of this method is insufficient for identifying low frequency mutations.

A simple and robust real-time qPCR method for the detection of PIK3CA mutations.

2018

Alvarez-Garcia V; et al... (2018)

Low sensitivity and the high cost.

Droplet Digital PCR for Mutation Detection in Formalin-Fixed, Paraffin-Embedded Melanoma Tissues: A Comparison with Sanger Sequencing and Pyrosequencing.

2018

McEvoy AC; et al. (2018)

Failure to detect mutations in genes and specific samples.

Implementation of next generation sequencing technology for somatic mutation detection in routine laboratory practice.

2018

Giardina T; et al. (2018)

It presents greater difficulties in detecting materials that were not microdissected.

Non-reproducible sequence artifacts in FFPE tissue: an experience report.

2017

Ofner R, et al. (2017)

Low reproducibility in FFPE.

Clinical validation of the 50 gene AmpliSeq Cancer Panel V2 for use on a next generation sequencing platform using formalin fixed, paraffin embedded and fine needle aspiration tumour specimens.

2017

Rathi V; et al. (2017)

Disadvantages in relation to new technologies such as NGS

Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis.

2016

Ishige T; et al (2016)

Sensitivity of this method is insufficient for identifying low frequency mutations.

Clinical Applications of Next-Generation Sequencing in Cancer Diagnosis

2016

Sabour L; et al. (2017)

Laborious, time consuming and difficulty in distinguishing between normal and altered genotypes.

A combination of immunohistochemistry and molecular approaches improves highly sensitive detection of BRAF mutations in papillary thyroid cancer.

2016

Martinuzzi C; et al. (2016)

Low sensitivity