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Table 3 Shows some recent articles arguing about advantages and disadvantages of Sanger sequencing in relation to other methods

From: KIT exon 11 and PDGFRA exon 18 gene mutations in gastric GIST: proposal of a short panel for predicting therapeutic response

Title Age Authors Argumentation
Comparison of an in vitro Diagnostic Next-Generation Sequencing Assay with Sanger Sequencing for HIV-1 Genotypic Resistance Testing. 2018 Tzou PL, et al. (2018) Sensitivity of this method is insufficient for identifying low frequency mutations.
A simple and robust real-time qPCR method for the detection of PIK3CA mutations. 2018 Alvarez-Garcia V; et al... (2018) Low sensitivity and the high cost.
Droplet Digital PCR for Mutation Detection in Formalin-Fixed, Paraffin-Embedded Melanoma Tissues: A Comparison with Sanger Sequencing and Pyrosequencing. 2018 McEvoy AC; et al. (2018) Failure to detect mutations in genes and specific samples.
Implementation of next generation sequencing technology for somatic mutation detection in routine laboratory practice. 2018 Giardina T; et al. (2018) It presents greater difficulties in detecting materials that were not microdissected.
Non-reproducible sequence artifacts in FFPE tissue: an experience report. 2017 Ofner R, et al. (2017) Low reproducibility in FFPE.
Clinical validation of the 50 gene AmpliSeq Cancer Panel V2 for use on a next generation sequencing platform using formalin fixed, paraffin embedded and fine needle aspiration tumour specimens. 2017 Rathi V; et al. (2017) Disadvantages in relation to new technologies such as NGS
Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis. 2016 Ishige T; et al (2016) Sensitivity of this method is insufficient for identifying low frequency mutations.
Clinical Applications of Next-Generation Sequencing in Cancer Diagnosis 2016 Sabour L; et al. (2017) Laborious, time consuming and difficulty in distinguishing between normal and altered genotypes.
A combination of immunohistochemistry and molecular approaches improves highly sensitive detection of BRAF mutations in papillary thyroid cancer. 2016 Martinuzzi C; et al. (2016) Low sensitivity